According to recent research from Edmonton, Canada, "In shotgun proteome analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS), not all coeluting peptides at a given retention time are subjected to MS/MS due to the limitation of spectral acquisition speed of a mass spectrometer. In this work, precursor ion exclusion (PIE) in an electrospray ionization (ESI) quadrupole time-of-flight (QTOF) mass spectrometer was explored as a means of mitigating the undersampling problem."
"This strategy is based on running replicates of the sample where the precursor ions detected in the initial run(s) are excluded for MS/MS in the subsequent run. Four PIE methods as …
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